Fig. 4

Protein enrichments and phosphorylation activity differences in males and females. A Histogram of Pearson correlation values of protein and RNA expression. Venn diagram shows a unique gene list that significantly correlated (p-value < 0.05, correlation > median) in each group. B DAVID Gene Ontology enrichment analysis for the significantly correlated unique gene list in males (top) and females (bottom). The data show the top 20 gene sets by p-value. The x-axis is log10 (annotated gene count). C Analysis of differentially expressed proteins and phosphorylated proteins in males and females with GBM. The cutoff for log2 fold change is 0.5 and the cutoff for p-value is 0.05. D Abundance of EGFR and phosphorylated EGFR proteins in males and females. E Abundance of SPP1 and phosphorylated SPP1 proteins in males and females. F Comparison of significant p-values for protein and phosphorylated protein to RNA in females and males. G EGFR Y1092-PLCG1 Y783 and EGFR Y119-CALM2 Y148 correlated expression. H 20 EGFR kinase and 16 substrates significant correlation. Turquoise is significant in males, pink is significant in females. I Cis–trans effects of mutated core genes (y-axis) in GBM on protein and phosphorylated protein levels (x-axis)