Fig. 6

Concentrations of SCFA in the feces of male and female FCG mice. Fecal pellets from naïve FCG mice were collected and analyzed for a panel of short-chain fatty acids: acetate (A), butyrate (B), propionate (C), 2-methylbutyric acid (D), hexanoic acid (caproic acid, E), isobutyric acid (F), and isovaleric acid (G) by LC–MS/MS (Metabolon). Data are represented as the mean ± SEM with each point representing one mouse. Statistics by two-way ANOVA with Tukey’s multiple comparisons test. Comparisons in graphs are representative of multiple comparisons tests; *p < 0.05; **p < 0.01. ANOVA main and interactive effects are provided in Additional file 9: Table S13