Fig. 3

KDM6a inhibition enhances plasma cell differentiation, but not IgM secretion, similarly in all four genotypes. Splenocytes isolated from naïve FCG mice were stimulated ex vivo with IL-4 (0.01 µg/mL) and LPS (5 µg/mL) in the presence or absence of 2 µM GSK J4 or 2 µM GSK J5. Representative density plots of flow cytometric data from naïve (day 0) or stimulated splenocytes (day 3) ± GSK J4 exposure are depicted in (A). Percentages of CD138 + plasma cells in FCG mice were quantified by flow cytometry (B). Supernatants were collected and total IgM concentrations were assessed by ELISA for each stimulation condition (C). Data are represented as the mean ± SEM with each data point representing one mouse. Three-way ANOVA with Tukey’s multiple comparisons tests. Comparisons in graphs are representative of multiple comparisons tests; **p < 0.01; ****p < 0.0001. ANOVA main and interactive effects are provided in Additional file 9: Table S6. Results from additional concentrations of GSK J4 and GSK J5 are provided in Additional file 5: Fig. S5 and Additional file 9: Table S7