Fig. 2

p-TrkB immunoactivity increased following OGD/REOX .ERα^+/+ and ERα^−/− hippocampal neurons were grown on coverslips and subjected to 4 h OGD followed by VC or 3 µM 7,8-DHF during 24 h REOX. Cells were fixed and stained with anti-p-TrkB and anti-MAP-2 antibodies. A. Representative images of male and female ERα^+/+ hippocampal neurons under normoxic conditions or after 4 h OGD followed by 24 h REOX with and without 7,8-DHF treatment (3µM). OGD/REOX resulted in increased p-TrkB staining in male and female hippocampal neurons. However, 7,8-DHF treatment resulted in a further increase in p-TrkB staining in female neurons, but not males. Inset: primary antibody control (p-TrkB and MAP-2). Arrow = co-localized p-TrkB and MAP-2 staining; Arrowhead = lack of p-TrkB staining. B. Summary figure of p-TrkB staining intensity in ERα^+/+ and ERα^−/− hippocampal neurons grown on coverslips and subjected to 4 h OGD followed by 24 h REOX with and without 7,8-DHF treatment (3µM). Data are mean ± SEM, ERα^+/+, n = 4–11; ERα^−/−, n = 6. Significance was determined by multi-factorial analysis of variance