Fig. 3

Sex-specific differences in vocalizations and vocalization latency in FXS Pups. (A) FXS pups of both sexes display a higher number of vocalizations compared to their control counterparts. (B) Only females show a shorter vocalization latency in the absence of FMRP. (A, B) Data are presented as min. to max. box plots with median and 25–75 percentile. Single dots represent individual mice. Mann-Whitney U tests were conducted, and p-values < 0.05 are indicated in the graphs. Full statistical details can be found in Suppl. Table 2. (C, D) Pie graphs illustrate the percentages of vocalizing (V) and non-vocalizing (NV) male (C) and female (D) pups. The percentages were calculated by dividing the number of vocalizers or non-vocalizers by the total number of animals tested in each group. Sample sizes: (A, C, D) +/y males N = 22, -/y males N = 21, +/+ females N = 12, +/- females N = 26, -/- females N = 6. (B) +/y males N = 17, -/y males N = 18, +/+ females N = 10, +/- females N = 20 and -/- females N = 6