Table 1 Primer sequence used for Real-Time polymerase chain reaction analysis
From: Male versus female inflammatory response after brain death model followed by ex vivo lung perfusion
Gene | Primers | Amplicon size (bp) |
|---|---|---|
β-actin | 5ʹ-GGAAATCGTGCGTGACATTAAA-3ʹ | 74 |
5ʹ-GCGGCAGTGGCCATCTC-3ʹ | ||
IL-1β | 5ʹ-CAGCAATGGTCGGGACATAGTT-3ʹ | 75 |
5ʹ-GCATTAGGAATAGTGCAGCCATCT-3ʹ | ||
IL-6 | 5ʹ-CCAACTTCCAATGCTCTCCTAATG-3ʹ | 89 |
5ʹ-TTCAAGTGCTTTCAAGAGTTGGAT-3ʹ | ||
CINC-1 | 5ʹ-TGGTTCAGAAGATTGTCCAAAAGA-3ʹ | 78 |
5ʹ-ACGCCATCGGTGCAATCTA-3ʹ | ||
eNOS | 5ʹ-AGTCCTCACCGCCTTTTCCA-3ʹ | 94 |
5ʹ-GCACGCGGTGAACCTCC-3ʹ | ||
C3 | 5ʹ-CAGCCTGAATGAACGACTAGACA-3ʹ | 93 |
5ʹ-TCAAAATCATCCGACAGCTCTATC-3ʹ |