Fig. 5
Effect of astrocyte-conditioned culture medium on Cho and APP levels in neuronal membrane rafts. A Cho levels in membrane rafts of neurons determined by HP-TLC followed by the analysis of intensity of bands (n = 12). B APP/flotillin ratio in neuronal membrane rafts determined by western blot (n = 12). All determinations were made after 24 h of neuronal primary culture exposure to conditioned astrocyte culture medium previously exposed or not (control; black bars) to 400 µM of CuSO4 (gray bar). Results were calculated using 2-way ANOVA plus Bonferroni’s multiple comparisons test, and data expressed mean ± SD, **p < 0.01 and ***p < 0.001 significant differences between Cu vs control, #p < 0.01 significant differences between Cu female vs Cu male