Table 2 Primers used for gut–microbiota abundance on cecal DNA with qPCR
Target Taxa | Sense | Oligonucleotides | Tm uses (°C) |
|---|---|---|---|
Eubacteria | Forward | ACTCCTACGGGAGGCAGCAG | 60 |
Reverse | ATTACCGCGGCTGCTGG | ||
Bacteroidetes (newly named Bacteroidota) | Forward | CATGTGGTTTAATTCGATGAT | 51 |
Reverse | AGCTGACGACAACCATGCAG | ||
Firmicutes (newly named Bacillota) | Forward | ATGTGGTTTAATTCGAAGCA | 51 |
Reverse | AGCTGACGACAACCATGCAC | ||
Prevotella | Forward | GGTTCTGAGAGGAAGGTCCCC | 60 |
Reverse | TCCTGCACGCTACTTGGCTG | ||
Lactobacillus | Forward | AGCAGTAGGGAATCTTCCA | 58 |
Reverse | CACCGCTACACATGGAG | ||
Limosilactobacillus reuteri | Forward | ACCGAGAACACCGCGTTATTT | 59 |
Reverse | ACCTAAACAATCAAAGATTGTCT | ||
Lactobacillus johnsonii/gasseri | Forward | CACTAGACGCATGTCTAGAG | 60 |
Reverse | AGTCTCTCAACTCGGCTATG | ||
Ligilactobacillus murinus/animalis | Forward | TCGAACGAAACTTCTTTATCACC | 60 |
Reverse | CGTTCGCCACTCAACTCTTT | ||
Verrucomicrobia | Forward | GAATTCTCGGTGTAGCA | 59 |
Reverse | GGCATTGTAGTACGTGTGCA | ||
β-Proteobacteria | Forward | GGGGAATTTTGGACAATGGG | 58 |
Reverse | ACGCATTTCACTGCTACACG | ||
δ-Proteobacteria | Forward | GGTGTAGGAGTGAARTCCGT | 62 |
Reverse | TACGTGTGTAGCCCTRGRC | ||
γ-Proteobacteria | Forward | CMATGCCGCGTGTGTGAA | 54 |
Reverse | ACTCCCCAGGCGGTCDACTTA | ||
Faecalibacterium prausnitzii | Forward | GATGGCCTCGCGTCCGATTAG | 60 |
Reverse | GATGGCCTCGCGTCCGATTAG | ||
Roseburia | Forward | GGTGTAAAGGGAGCGCAGG | 60 |
Reverse | ACTTACCCCTCCGACACTCT | ||
Clostridium cocleatum | Forward | GTAATACATAAGTAACCTGGCCTTT | 60 |
Reverse | CTCGGATGTCATTTCCTCC | ||
Akkermansia muciniphila | Forward | CAGCACGTGAAGGTGGGGAC | 60 |
Reverse | CCTTGCGGTTGGCTTCAGAT |