Sex-dependent circadian alterations of both central and peripheral clock genes expression and gut–microbiota composition during activity-based anorexia in mice

Table 1 Primers used to study circadian rhythm by RT-qPCR

Target gene	Sense	Oligonucleotides	Tm uses (°C)
Gapdh	Forward	CATCACTGC CACCCAGAAGA	60.0
	Reverse	AAGTCG CAG GAG ACA ACC T
	Reverse	TTCCTCAACACCACATGAGC
Bmal1	Forward	CACCGTGCTAAGGATGGCTG	55.4
Bmal1	Reverse	CTGCTGCCCTGAGAATTAGG	55.4
Clock	Forward	GGACACGGATGATAGAGGCA	56.3
Clock	Reverse	GTATCATGTGCTGGCCTGTG	56.3
Rev-erbα	Forward	CCCTGGACTCCAATAACAACACA	61.0
Rev-erbα	Reverse	GCCATTGGAGCTGTCACTGTAG	61.0
Per1	Forward	CCAGATTGGTGGAGGTTACTGAGT	61.0
Per1	Reverse	GCGAGAGTCTTCTTGGAGCAGTAG	61.0
Per2	Forward	TTCCACTATGTGACAGCGGAGG	61.0
Per2	Reverse	CGTATCCATTCATGTCGGGCTC	61.0
Cry1	Forward	GGAAGGAACGAGATGCAGCT	59.4
Cry1	Reverse	AGTGGCTCCATCTTGCTGAC	59.4
Cry2	Forward	GGGACTCTGTCTATTGGCATCTG	57.1
	Reverse	GTCACTCTAGCCCGCTTGGT
	Reverse	AGAGGTGGTGTAAGCCATGC

ISSN: 2042-6410