Fig. 7
Neutrophils and macrophage subsets in liver in males and females of Balb/c or CD1d−/− mice after Chow or HFCD feeding. a Neutrophils (Ly6G+); b MPO immunohistochemistry (20X), red arrows point to MPO-positive cells in male tissue from sections from 6 weeks HFCD-fed Balb/c (WT) or CD1d-KO mice; c macrophages (F4/80+, CD11b+); d Ly6Chi macrophages; e Ly6Clo macrophage; or f ratio Ly6Chi/Ly6Clo macrophages were determined by flow cytometry in hepatic tissue of Chow or 6 weeks HFCD-fed Balb/c (WT) or CD1d-KO mice, males or females; g representative F4/80 immunohistochemistry (20x), in 6 weeks HFCD-fed Balb/c (WT) or HFCD-fed CD1d-KO male mice, red arrows indicated hepatic crown-like structures in male tissue sections from HFCD Balb/c (WT) or CD1d-KO mice. Data are expressed as mean ± SEM, n = 4–5, *p < 0.05, **p < 0.005, ***p < 0.001 vs. corresponding compared group. To compare the means between two particular groups the Student’s t test will be used, and the resulting p value expressed numerically in the figure