Fig. 1

Sex-selective changes in K48-polyubiquitin protein targets in the amygdala of male and female rats across development. The basolateral amygdala (BLA) of 4-week-old (4 wk) and 9-week-old (9 wk) male and female rats was collected (n = 10 animals per group per sex per age). A subset of the samples was used for liquid chromatography mass spectrometry (LC/MS) to identify K48-polyubiquitin protein targets in whole cell protein lysates purified with a K48-specific tandem ubiquitin binding entity (K48-TUBE) (n = 6 animals per group per sex per age). The log₂(Protein Abundance + 1) values are plotted for proteins identified as being targeted differently by K48-polyubiquitination across development in females (A) and males (B). C List of the proteins in both sexes that had increased or decreased levels in K48-polyubiquitin-purified samples across development in females (blue) and males (gray) with their corresponding Log₂(Fold Change) at 4 wk compared to 9 wk. Data were a sum of two technical replicates. Log₂(Fold Change) was calculated by adding 1 to every value (Protein Abundance + 1), taking the average of the groups and then dividing the appropriate groups for each comparison followed by log₂ transformation of the resulting values