Fig. 4

Both sexes display frontloading behavior in alcohol but not water consumption. A-E) EtOH and water consumption in females during the first vs. second 2 h of Day 4 for 20% EtOH, 10% EtOH, and H2O DID. A Females consumed more 20% EtOH (g/kg) during the first vs. second 2 h of Day 4 DID on cycles 2 and 4–7. B Females consumed more 10% EtOH (g/kg) during the first vs. second 2 h of Day 4 DID on all cycles. C–E There was no difference in female H2O consumption (ml/kg) during the first vs. second 2 h in 20% EtOH DID (C), 10% EtOH DID (D), or H2O DID (E). F–J EtOH and water consumption in males during the first vs. second 2 h of Day 4 for 20% EtOH, 10% EtOH, and H2O DID. F Males consumed more 20% EtOH (g/kg) during the first vs. second 2 h of Day 4 DID on cycles 2–4 and 6. G) Males consumed more 10% EtOH (g/kg) during the first vs. second 2 h of Day 4 DID on all cycles. H–J There was no difference in male H2O consumption (ml/kg) during the first vs. second 2 h in 20% EtOH DID (H), but consumption was higher on the first vs. second 2 h for 10% EtOH DID (I) and H2O DID (J). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 in 2xRM-ANOVA main effects and interactions of time point and DID cycle, as well as post hoc t-tests with H–S corrections between the first and second 2 h