Fig. 3

Higher female water consumption emerges across the day. A–C Water consumption at 2-h, 4-h, and 24-h time points on Day 4, 2-bottle choice (2-BC) access in H2O DID across cycles. A There were no sex differences in H2O consumption (ml/kg) during the first 2 h of Day 4 access. B Females consumed more H2O (ml/kg) than males during the first 4 h of Day 4 on cycles 3 and 8, with a trend towards increased consumption during cycle 6. C Females consumed more H2O (ml/kg) than males during the 24-h period on cycles 2–8. D–F Average total volume consumption for Day 4 EtOH and water in 20% EtOH, 10% EtOH, and H2O DID. D During the first 2 h, total volume consumption was highest for 10% EtOH, and females consumed more fluid than males in 10% EtOH DID. E Within 4 h, females consume greater volume than males for all conditions, and consumption was higher for H2O, particularly in females. F At the 24-h time point, females consumed more fluid than males in all DID conditions; there were no differences in fluid consumption between DID conditions in either sex. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 in 2xRM-ANOVA main effects and interactions of sex and DID cycle (A–C) and 2xANOVA main effects of sex and EtOH concentration (D–F), as well as post hoc t-tests with H–S corrections as indicated. ^$P < 0.10 for post hoc t-tests with H–S corrections between M and F