Fig. 1

Alcohol and water consumption are sex- and alcohol concentration-dependent. A Schematic depicting the modified Drinking in the Dark (DID) binge drinking paradigm in C57BL/6J mice. B Bodyweight gain was similar across DID cycles in H2O, 10% EtOH, and 20% EtOH DID mice of both sexes. C Cumulative intake of 20% EtOH solution (ml) was higher in females compared to males on DID cycles 5–8. D There was no sex difference in cumulative 10% EtOH intake (ml) across DID cycles. E There was no sex difference in cumulative H2O intake (ml) across DID cycles. F Cumulative EtOH consumption normalized to bodyweight (g/kg) was higher in females compared to males on DID cycles 3–8. G Females consumed more 10% EtOH (g/kg) compared to males on DID cycles 4–8. H Females consumed more H2O (ml/kg) compared to males during DID cycles 6–8, with a trend on cycle 5. I Females displayed higher average 20% EtOH consumption (g/kg) during the first 2 h /day of access on DID cycles 2–8. J Females displayed higher average 10% EtOH consumption (g/kg) on the first 2 h /day of access on DID cycles 3, 4, and 6. K There was no difference between males and females on average H2O consumption during the first 2 h/day of water access. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 in 2xRM-ANOVA main effects and interactions of sex and DID cycle, as well as post hoc t-tests with H–S corrections between M and F. ^$P < 0.10 for post hoc t-tests with H–S corrections between M and F