Fig. 9

Effect of estradiol (E2) in the formation of neurospheres and neurogenesis from SVZ-isolated cells in the adult prairie vole. Number of neurospheres formed (a, c, e, g) and their diameter (b, d, f, h) twelve days after the start of culture with E2 treatments. Percentage of MAP2 + (i, k, m, o) and GFAP + (j, l, n, p) cells in neurospheres’s cell outgrowth cultured for fifteen days under adherent conditions. E2 treatments: 0.5 μM, 1 μM, 2 μM and 1 μM with EGF (20 ng/mL) co-treatment. Data were analyzed with a two-way ANOVA followed by Tukey’s post hoc test. n = 4 subjects per group of each sex. SCM different from Co in the same sex, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Female different from male in the same sociosexual condition, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001