Fig. 5

Cells isolated from SVZ have a greater potential for differentiation towards neuronal lineage and a decrease towards glial lineage in sociosexual voles. a and b Representative fluorescence microscopy images of mature neurons MAP2 + (red) and glial GFAP + (green) cells from SVZ-derived neurospheres of control (Co), social exposure (SE) and social cohabitation with mating (SCM) groups in female (Fe) and male (Ma) adult voles. Nuclei were stained with DAPI (blue). Scale bars = 50 µm. b Determination of immunoreactivity to MAP2 in differentiated cells from SVZ-derived neurospheres of Co, SE and SCM groups in both Fe and Ma adult voles. Females n = 10 per group, MaCo and MaSE (n = 10) and MaSCM (n = 9). c Quantification of immunoreactivity to GFAP in differentiated cells from SVZ-derived neurospheres of Co, SE and SCM groups. FeCo and FeSCM (n = 10 per group), FeSE (n = 9); MaCo and MaSE (n = 10) and MaSCM (n = 9). The MAP2 and GFAP data for each sex group (Fe or Ma) was analyzed with a one-way ANOVA followed by Tukey’s post hoc test. Six neurospheres per individual were analyzed. Different from Co group **p < 0.005, ****p < 0.0001