Fig. 4

Social exposure affects DCX and migration in cells derived from neurospheres grown up from SVZ-isolated adult voles. a Representative fluorescence microscopy images of DCX + (green) cells in the outgrowth of attached SVZ-derived neurospheres of control (Co), social exposure (SE) and social cohabitation with mating (SCM) groups in both female (Fe) and male (Ma) adult voles. Nuclei were stained with DAPI (blue). Scale bars = 100 µm. b Relative immunofluorescence of DCX + cells in SVZ-derived neurospheres. The data for each sex group was analyzed with a one-way ANOVA followed by Tukey’s post hoc test. FeCo, FeSCM (n = 8 per group), FeSE (n = 7); MaCo and MaSCM (n = 8 per group) and MaSE (n = 7). Six neurospheres per individual were analyzed. c Migration (measured distance from the edge of the neurospheres to the cell outgrowth) of cells from SVZ-derived neurospheres. To measure relative migration, light phase-contrast photomicrographs were taken and the average distance between the radius of the inner and outer circumference of the neurosphere outgrowth was calculated. Data was analyzed with a two-way ANOVA followed by Tukey´s post hoc test. n = 6 per group in each sex, six neurospheres per individual were analyzed. Different from Co group ***p < 0.001, ****p < 0.0001. Sex differences in the same group ^#p < 0.05, ^####p < 0.0001