Fig. 3

Increased proliferation in the cellular outgrowth of attached neurospheres derived from SVZ-isolated cells in the adult vole previous sociosexual stimuli. a Representative fluorescence microscopy images of Nestin + (green) cells from SVZ-derived neurospheres of control (Co), social exposure (SE) and social cohabitation with mating (SCM) groups in both female (Fe) and male (Ma) adult voles. Nuclei were stained with DAPI (blue). Individual nuclei are not visible because neurospheres can comprise several hundred cells. Scale bars = 50 µm. b Determination of immunoreactivity to Nestin in undifferentiated cells from SVZ-derived neurospheres. Relative fluorescence was analyzed with a one-way ANOVA followed by Tukey’s post hoc test. FeCo and FeSE (n = 8 per group) and FeSCM (n = 9); for males n = 9 per group. Six neurospheres per individual were analyzed. c Representative fluorescence microscopy images of EdU + (green) and Nestin + (red) cells from SVZ-derived neurospheres of Co, SE and SCM groups in both female (Fe) and male (Ma) adult voles. Nuclei were stained with DAPI (blue). Scale bars = 50 µm. d Percentage of EdU + cells in undifferentiated cells from SVZ-derived neurospheres. The data was analyzed with a one-way ANOVA followed by Tukey’s post hoc test. Females n = 10 per group; MaCo and MaSCM (n = 10 per group) and for MaSE (n = 9). Six neurospheres per individual were analyzed. Different from the Co group *p < 0.05, **p < 0.01 and ***p < 0.001. Sex differences in the same sociosexual group ^#p < 0.05, ^##p < 0.01