Fig. 2

Social exposure and social cohabitation with mating increase the number and size of SVZ-derived neurospheres from adult prairie voles. a Representative micrographs of neurospheres derived from SVZ-isolated cells of the adult female (Fe) and male (Ma) prairie vole in control (Co), social exposure (SE) and social cohabitation with mating (SCM) groups. Scale bar, 200 µm. b Number of neurospheres obtained from SVZ in both Fe and Ma voles. Data were analyzed with a two-way ANOVA followed by a Tukey’s post hoc test. n = 7 subjects per group of each sex. c Size (diameter) of neurospheres measured 12 days after initiation of culture. Data were analyzed with a two-way ANOVA followed by Tukey’s post hoc test. n = 6 per group of each sex, seven neurospheres per individual were analyzed. Different from Co group *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Sex differences in the same sociosexual group ^###p < 0.001, ^####p < 0.0001