Fig. 12

Effect of progesterone (P4) in the formation of neurospheres and neurogenesis from SVZ-isolated cells in the adult prairie vole. Number of neurospheres formed (a–c) and their diameter (d–f) twelve days after the start of culture with P4 treatments. Percentage of MAP2 + (g–i) and GFAP + (j–l) cells in neurospheres cultured for fifteen days under adherent conditions. P4 treatments: 1 μM, 2 μM, and 1 μM with EGF (20 ng/mL) co-treatment. Data were analyzed with a one-way ANOVA followed by Tukey’s post hoc test. n = 4 subjects per group of each sex. SCM different from Co in the same sex, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Female different from male in the same sociosexual condition, ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001