Fig. 10

Effect of prolactin (PRL) in the formation of neurospheres and neurogenesis from SVZ-isolated cells in the adult prairie vole. Number of neurospheres formed (a–d) and their diameter (e–h) twelve days after the start of culture with PRL treatments. Percentage of MAP2 + (i–l) and GFAP + (m–p) cells in neurospheres cultured for fifteen days under adherent conditions. PRL treatments: 50 ng/mL, 100 ng/mL, 200 ng/mL and 100 ng/mL with EGF (20 ng/mL) co-treatment. Data were analyzed with a two-way ANOVA followed by Tukey’s post hoc test. n = 4 subjects per group of each sex. SCM different from Co in the same sex, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Female different from male in the same sociosexual condition, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001