Fig. 2

Gal-3 is associated with inflammation in men and women AVs. Positive correlations between Gal-3 and IL-6 (A), CCL-2 (B), RANTES (C), ICAM-1 (D), CD44 (E) and CD68 (F) in AVs from AS patients. Representative microphotographs of AV sections from AS patients immunostained for Gal-3 along with CD68 (G), CD206 (H), CD45 (I) and CD80 (J). Protein levels of IL-6 (K) and CCL-2 (L) measured by ELISA after stimulation in vitro with Gal-3 and with MCP or G3P-01. IL interleukin, CCL-2 C–C motif chemolike ligand 2, ICAM-1 Intercellular Adhesion Molecule 1, CD cluster of differentiation, Gal-3 galectin-3. N = 108 AVs from men and N = 83 AVs from women. In vitro: number of biological replicates: 12 men and 12 women; number of technical replicates: 3–6