Fig. 3

SON gene expression comparisons. A Euler diagram demonstrating that the 2097 genes identified by the Loupe Browser (conservative approach, genes with low average counts excluded) in the SON of male and/or female rats are among the 17,225 genes identified by DESeq2 in our Visium samples (males, n = 4; females, n = 4). B Data demonstrate a robust positive correlation between Loupe Browser (conservative approach) gene average and DESeq2 normalized counts in the SON for 2097 commonly discovered RNAs. C Data demonstrate a robust positive correlation between Loupe Browser (less conservative approach, genes with low average counts included) gene average and DESeq2 normalized counts in the SON show 100% of common genes identified by both methods. D Venn diagram demonstrating the overlapping of SON transcripts discovered by Visium in male rats in comparison with the previously published bulk SON RNAseq [19] and bulk SON proteome [48] data in control male rats. E Positive correlation between Visium and bulk SON RNAseq [19] data in control male rats. F Positive correlation between Visium and bulk SON proteome [48] data in control male rats