Sexual differences in locus coeruleus neurons and related behavior in C57BL/6J mice

Table 1 Summary of statistical analysis of LC-related anxiety and depressive-like behaviors

Shapiro–Wilk normality test (W, P value, Passed normality test?)
	Male				Female
Time in open arms (%)	0.9234 0.3865 Yes				All 0.8643 0.0857 Yes		P/E 0.9315 0.5639 Yes		D/M 0.8406 0.2155 Yes
Time in lit compartment (%)	0.8558 0.0864 Yes				0.9143 0.4264 Yes
Time in center (%)	0.9206 0.3617 Yes				0.9046 0.2456 Yes
Material burrowed (g)	1 h 0.9296 0.5120 Yes	3 h 0.8623 0.1266 Yes	6 h 0.8313 0.0613 Yes	24 h 0.4623 < 0.0001 No	1 h 0.5049 < 0.0001 No	3 h 0.4993 < 0.0001 No		6 h 0.8261 0.0404 No		24 h 0.8238 0.0381 No
AUC (Material burrowed)	0.9049 0.3197 Yes				0.9196 0.3887 Yes
Grooming time (s)	0.8880 0.1611 Yes				0.9472 0.6354 Yes
TST Immobility time (s)	0.9268 0.4172 Yes				0.9310 0.4580 Yes
FST Immobility time (s)	0.9173 0.3349 Yes				0.8900 0.1698 Yes
EPM Total activity (AU)	0.9425 0.5806 Yes				0.9622 0.8109 Yes
Entries in open arms	0.9464 0.6265 Yes				0.9103 0.2833 Yes
Latency to dark compartment (s)	0.8811 0.1612 Yes				0.8739 0.2005 Yes
Number of transitions	0.9165 0.3644 Yes				0.9380 0.6209 Yes
OF Total distance (AU)	0.8886 0.1636 Yes				0.9109 0.2872 Yes
Latency to groom (s)	0.9813 0.9717 Yes				0.8561 0.0686 Yes
Latency to immobility (s)	0.8723 0.1063 Yes				0.9226 0.3793 Yes

Unpaired Student’s t-test (t_(df))
Time in open arms (%)	t₍₁₈₎ = 3.06**
Time in lit compartment (%)	t₍₁₄₎ = 2.32*
Time in center (%)	t₍₁₈₎ = 2.16*
AUC (Material burrowed)	t₍₁₅₎ = 3.86**
Grooming time (s)	t₍₁₈₎ = 0.28
TST Immobility time (s)	t₍₁₈₎ = 0.99
FST Immobility time (s)	t₍₁₈₎ = 0.36
EPM Total activity (AU)	t₍₁₈₎ = 1.99
Entries in open arms	t₍₁₈₎ = 0.34
Latency to dark compartment (s)	t₍₁₄₎ = 0.07
Number of transitions	t₍₁₄₎ = 0.55
OF Total distance (AU)	t₍₁₈₎ = 0.85
Latency to groom (s)	t₍₁₈₎ = 3.69**
Latency to immobility (s)	t₍₁₈₎ = 0.46

One-way ANOVA (F_{(df. residual)})
Time in open arms by estrous cycle (%)	F_(2,17) = 12.32***

Repeated-measures ANOVA (F_{(df. residual)})
	Sex	Time	Sex × Time
Material burrowed (g)	F_{(1, 15)} = 11.08**	F_{(3, 45)} = 39.14***	F_{(3, 45)} = 7.12***

P/E, Proestrus/Estrus; D/M, Diestrus/Metestrus; AUC, area under the curve; TST, tail suspension test, FST, forced swimming test; EPM, elevated plus maze; OF, open field; AU, arbitray units; df, degrees of freedom
*p < 0.05; **p < 0.01; ***p < 0.001
Values in bold indicate statistically significant results

ISSN: 2042-6410