Fig. 7
Treatment with dasatinib cleared senescent cells from PE-MSC and affected senescence-related gene expression. Representative images from SABG staining show abundant senescent cells in PE-MSC (marked with black arrows), but not in NP-MSC, in vehicle (a). Dasatinib treatment completely removed senescent cells from PE-MSC (p < 0.001) (b). PE-MSC had a significant decrease in expression of p16 (p = 0.025) and PAI-1 (p < 0.001), IL-6 (p = 0. 0487), and MCP-1 (p = 0.040), while IL-8 (p = 0.136) was modestly decreased after treatment. Significantly increased expression of the p21 and PAI-2 genes was observed after the treatment (c). Relative expression of the senescence marker gene, p16, in NP-MSC after treatment with Dasatinib remained unchanged (p = 0.136). Apart from p21 and PAI-2 whose expression increased in a manner similar to PE-MSC, the relative gene expression of the other tested genes was decreased in NP-MSC after the treatment with dasatinib (p < 0.001) (d)